Fig. 7. Notch represses Twist by regulating Emc activity. (A,C,E) Whole-mount embryos. (B,D,F) Corresponding close-ups of embryos in (A,C,E). (A-D) Lateral views of stage 11 embryos stained with anti-Emc. (A,B) Wild-type (wt) embryo shows strong Emc expression around its ectodermal tracheal pits (black arrowheads) and little or no mesodermal Emc expression (white asterisks). (C,D) UAS-N^intra embryo expresses Emc both around its tracheal pits (black arrowheads) and throughout its mesoderm (white asterisks). (E,F) Lateral views of a stage 10 N^null; UAS-emc embryo stained with anti-Twist. Emc represses Twist in N^null mutants such that Twist is expressed in low and high domains; compare with Fig. 1E,F and Fig. 3C-J.