Fig. 1. Alignment of Mixer morpholinos against Mixer and related Xenopus sequences (A) and morpholino inhibition of in vitro translation (B) of wild-type and MO-R Mixer mRNA. (A) Xenopus Mixer aligned against the reverse complement of the three morpholinos used in this study using Clustal alignment algorithem in Macvector. These sequences were also compared to a potential pseudo-allele identified by the Sanger EST sequencing project and the most closely related homeodomain proteins Mix.1 and Mix.2. (B) Mixer MO-1, MO-2 and MO-3 block translation of wild type but not MO-R Mixer mRNA in an in vitro translation assay as described in the Materials and methods.