Fig. 2. Non-complementary Mixer mRNA rescues the effects of Mixer depletion. Gastrulation is delayed in embryos injected with MO-1 (A) and MO-3 (B) as marked by formation of the dorsal blastopore lip. The gastrulation delay is partially rescued by the injection of Mixer MO-R mRNA into the four vegetal cells of eight-cell MO-1-injected embryos (C). Anterior and gut abnormalities in Mixer-depleted embryos is partially rescued by injection of Mixer MO-R mRNA into the four vegetal cells of the eight-cell embryo (D).