Fig. 5. Mixer-depleted vegetal masses have increased mesoderm inducing activity in Nieuwkoop recombinants. (Top) Schematic of real-time RT-PCR of Nieuwkoop recombinant experiment in which the capacity of wild-type and Mixer-depleted vegetal masses to induce mesoderm in animal caps is compared. eomesodermin, Fgf8, Xbra and Xnr1 are induced more efficiently in animal caps by Mixer-depleted vegetal masses than control vegetal masses. we, wild-type embryos; WT base-treated caps, 10 animal caps co-cultured with wild-type vegetal masses; MO-1 base-treated caps, 10 animal caps co-cultured with Mixer-depleted vegetal masses. Expression levels were normalized to ODC.