Fig. 3. Production of NO in 7.5 dpc conceptuses. NO production and localization were preformed using DAF-FM. (A) Phase-contrast image of a 7.5 dpc conceptus showing the intact mesoderm (outlined by the broken red line) and the removed endoderm. (B) DAF-FM fluorescence (green) of the corresponding area. (C) Phase-contrast image of an enlargement of the boxed area in A containing the endodermal layer. (D) DAF-FM fluorescence of the corresponding area. (E) Representative western blots of eNOS and p-eNOS from eNOS immunoprecipitates of 8.5 dpc yolk sac lysates. (F) Representative western blots of Akt and p-Akt from 8.5 dpc yolk sacs.