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Fig. 4. Inhibition of PKA does not activate Hh target genes. (A-C) Injection of 250 pg of dnPKA mRNA into wild-type embryos induced the ectopic expression of myod at 12 hpf (A), ptc1 at 30 hpf (B) and nk2.2at 30 hpf (C). (D-F) In igu embryos, overexpression of dnPKA did not induce the expression of myod (D), nk2.2 (E) and ptc1 (F), and Hh target gene expression is similar to that in igu mutants. Genotypes were confirmed by PCR after in situ analysis.