Fig. 6. The zinc-finger gene dzip1 is disrupted in igu mutants. (A) Genetic map of igu mutation relative to known genes in the region. P1 artificial chromosome (PAC) clones (below) and predicted ORFs (arrows in the middle) are shown. (B) Sequence ferograms showing nonsense mutations in igu^ts294e and igu^tm79a alleles. (C) Schematic view of the structure of predicted Igu/Dzip1 protein. A conserved domain (yellow box) has 51% identity with the human DZIP1. Putative nuclear localization signal (NLS), zinc finger (red boxes) and PEST domains (blue boxes) are shown. (D) Amino acid sequence of zebrafish Igu/Dzip1 protein. The NLS (gray), zinc finger (yellow) and positions of nonsense mutations (green) are highlighted. The N-terminal conserved domain is boxed. PEST sequences are underlined.