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Fig. 7. Subcellular localization of Igu/Dzip1 proteins in cultured cells. (A-C) Transfection of a construct encoding the His-tagged Igu/Dzip1 protein into NIH3T3 cells. Wild-type Igu/Dzip1 proteins (A) are located in the cytoplasm and enriched in punctate vesicles. These vesicles are also enriched with the lysosomal protein Lamp1 (B, C). (D-F) Transfection of a construct encoding the His-tagged Igu/Dzip1(ts294e) mutant protein. The truncated mutant Igu/Dzip1 proteins (D) are distributed in the cytoplasm and enriched in nuclei. (C,F) Merged images, in which nuclei are stained with 4',6-diamidino-2-phenylindole (DAPI).