Fig. 8. Rescue of igu mutant phenotype by igu/dzip1 mRNA and permissive nature of Igu/Dzip1 proteins. (A-J) Injection of wild-type igu/dzip1 mRNA. Nearly all igu embryos injected with wild-type igu/dzip1 mRNA completely recovered the normal expression of Hh target genes (A-E), including: ptc1 expression at 14 hpf (A), nk2.2 expression at 30 hpf (B), ptc1 expression at 30 hpf (C, D) and en1 at 30 hpf (E) (compare with Fig. 1). Wild-type embryos injected with wild type igu/dzip1 mRNA did not show any defects in Hh target gene expression (F-J). (K-T) Injection of mutant mRNA that encodes truncated igu/dzip1 proteins. Expression of mutant Igu/Dzip1 does not affect Hh target gene expression in igu mutants (K-O) or wild-type embryos (P-T). (A,D,F,I,K,N,P,S) Dorsal views and other panels are side views. Expression patterns in A-J are identical to those seen in wild-type embryos, while those in K-O are identical to igu mutant embryos (see Fig. 1 for comparison).