Fig. 5. Developmental abnormalities of wrn-1(RNAi) worms and their penetrance influenced by growth temperature and ionizing radiation. (A) Morphological phenotypes of F1 progeny produced by P0 C. elegans worms microinjected with dsRNA of wrn-1. Small body, shorter than 70% of the wild-type body length and thin; dumpy, short and fat; ruptured body, gonad and intestine bursting out of the worm; bag of worms, hatched worms inside the adult worm due to a blockage of egg-laying; growth arrest, at various larval stages. Scale bars: 100 µm. (B-D) Frequency of wrn-1(RNAi) phenotypes. Wild-type N2 and F1 wrn-1(RNAi) worms were grown at (B) 20°C and (C) 25°C, and phenotypes were scored up to the 8-day adult stage. (D) Wild-type N2 and F1 wrn-1(RNAi) worms were -irradiated at 60 Gy and 20°C and their phenotypes scored after 3 days. Over 500 F1 worms were used in each measurement for B and C, and over 200 worms for D. Each experiment was repeated three times and standard errors (s.e.m.) are shown by error bars. Note that the % phenotype scale in D is different from that in B and C.