Fig. 6. DNA content in prespore and prestalk cells of migrating slugs. (A) Wild-type cells (AX4) marked with a prestalk reporter gene (ecmA/gfp) were developed on agar to the slug stage and dissected into two fractions enriched in anterior or posterior cells. (B) Cells were dissociated, fixed and stained with propidium iodide to obtain population profiles of their DNA content. (C) The cells were divided into GFP-negative (window A) and GFP-positive (window B) populations that were confirmed by direct visual inspection of sorted cells. The percentage of the total cell population in each window is shown. (D) The DNA content profiles of the GFP-negative cells in window A of the slug posteriors (upper panel) and the GFP-positive cells in window B of the anterior cells (lower panel).