Fig. 2. pbl mutant embryos have reduced numbers of EVE-positive mesodermal cells. (A,B) Anti-Fasciclin 3 stains and (C,D) anti-EVE stains of wild-type (A,C) and pbl²/pbl³ mutant embryos (B,D). (A) At stage 12 in a wild-type embryo, the visceral mesoderm is seen as a band of cells running along the anteroposterior axis (arrow). (B) In a similarly staged pbl²/pbl³ mutant embryo, the visceral mesoderm is evident, although it is less organised (arrow). (C) At stage 11 in a wild-type embryo, EVE is expressed in segmentally repeated clusters of dorsal mesodermal cells. The EVE-expressing neuroblasts are out of focus in this image. (D) In a similarly staged pbl²/pbl³ mutant embryo, the majority of EVE-positive mesodermal cells fail to form. One EVE-positive hemisegment can be seen in this embryo (arrow). Based on their position and morphology, the remainder of the EVE-expressing cells are neuroblasts (arrowheads).