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Fig. 7. The GEF function of PBL is required for the transition of the mesoderm from an epithelium to migratory cells during Drosophila gastrulation. (A,B) The majority of EVE-positive hemisegments fail to form in UAS-pbl{Delta}DH; pbl3/prd-GAL4, pbl2 (A) and pbl5/pbl5 embryos (B). (C) Cross-section of a stage 10 pbl5/pbl5 embryo stained for F-actin showing a typical failure of the mesoderm to disaggregate and spread dorsally. (D,E) UAS-GFP-Actin/twist-GAL4; pbl5/pbl5 embryos show similar morphology to UAS-GFP-Actin/twist-GAL4; pbl2/pbl3 embryos (compare with Fig. 5D,F). Scale bars: 10 µm.