Fig. 2. Hh signaling is required for function of the D-h activation element. Clones lacking smo function were examined in a prepupal leg, 3 hours APF. (A) HHRE-GFP expression (green). (B) myc expression (red). Homozygous smo^IIG26 clones are identified by the lack of Myc expression. (C) Merge of images in A and B (several smo-clones that overlap the HHRE-GFP stripe are outlined in white). Note the absence HHRE-GFP expression in smo^-clones