Fig. 7. Brk represses D-h expression. Clones lacking brk function were examined in a prepupal leg, 3 hours APF (A-C) and in a third instar leg disc (D-F). brk^– clones exhibit ectopic GFP expression anterior to the D-h-GFP stripe (A-C) and in the ventral leg (D-F). (A,D) D-h-GFP expression (small nuclei, green). Clones are outlined in white in all panels. White line marks the anterior boundary of D-h-GFP expression in A-C. Large GFP-expressing nuclei (arrowheads) probably correspond to adepithelial cells. (B,E) myc expression (red). brk^– clones are marked by the loss of myc expression. (C) Merge of images in A and B. (F) Merge of images in D and E.