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Fig. 8. A Brk response site in the D-h repression element is not sufficient for D-h repression. (A) Sequence of CMB element and CMB mutants: Mad2 (M2), Brk/Mad2 (BM2), Mad1/Mad2 (MM), Mad1/Brk/Mad2 (MBM), CRE/Brk (CB), CRE (C) and Mad1/CRE/Mad2 (MCM). Mutagenized bases are shown in lower case. (B-G) D-h-GFP directed by D-h-CMB site mutants: M2 (B), BM2 (C), MM (D), MBM (E), CB (F) and C (G). The insets in all panels show GFP expression in the basitarsal segment of a 4-hour prepupal leg.