Fig. 2. Ecdysoneless is conserved throughout eukaryotes. Alignment of the Drosophila (Dm) Ecd protein (Swiss-Prot Q9W032) with human (Hs) SGT1 (O95905), and its closest relatives from Arabidopsis thaliana (At; Q9LSM5) and Schizosaccharomyces pombe (Sp; Q9US49), using Clustal W. Black shading indicates amino acid identity, and gray shading indicates similarity if present in two or more of the aligned sequences. The putative ATP/GTP-binding site (P-loop) is marked by the horizontal arrow. Asterisks indicate the positions of the premature termination codons in alleles ecd², ecd^g24 and ecd^l(3)23. The arrowhead indicates the conserved proline 656, which is mutated to serine in ecd¹.