Fig. 5. Developmental expression of ecd. (A) Northern blot analysis showing expression of an ecd mRNA in embryos (E), in first (L1), second (L2) and third (L3) instar larvae, in wandering larvae (W), in white puparia (WP), in pupae (P) and in egg-laying females (add female symbol). Bottom panel shows a control re-hybridization of the blot with the constitutively expressed gene mbf1. (B) Western blot with the anti-Ecd antibody detects Ecd in unfertilized eggs from virgin mothers (Unf) and embryos 0-2 hours and 12-24 hours after egg laying.