Fig. 7. Ecd-deficient cells do not survive in proliferating imaginal discs. ecd²/ecd² (top row) and mbf1/mbf1 (bottom row) null mutant clones were induced during the first larval instar. Only the sister ecd^+/+ clones, marked by the intensive expression of P[w⁺, ub-GFP], are found in the adult eye (A), eye-antennal discs (B) and wing discs (C), whereas ecd^– cells are absent. Clones lacking mbf1, P[w⁺, ub-GFP] are maintained in all these imaginal tissues (D-F, arrows).