Fig. 8. Egg chambers carrying clones of ecd²/ecd² follicle cells fuse together. The normal number of 15 nurse cells in a control vitellogenic egg chamber (A) frequently doubles (B) when ecd^– clones are induced in adult females. Presence of two (C,D) or more (F) oocytes confirms egg chamber fusions. Fasciclin III expression appears at the ends of a double egg chamber, but not at the fusion line (E). Multiple FasIII signals may result from a fusion of several cysts (G). (A,A') Images show the same wild-type egg chamber. (D,D',E,E') Images show Orb, FasIII and Ecd proteins in the same egg chambers. Large clones of ecd^– cells are clearly visible (D') and smaller clones are emphasized with yellow lines (E'). Except for DNA staining with DAPI (A,B), all images are confocal sections. Arrows indicate Orb-positive oocytes; arrowheads indicate FasIII-positive polar follicle cells. Anterior is to the left.