Fig. 9. Loss of ecd in the germline causes previtellogenic arrest of egg chambers. (A,B) ecd²/ecd² germline clones (arrowheads) can be distinguished from an ecd^+/+ clone (arrow) by the lack of staining with the anti-Ecd antibody. All of these clones arrest because of the loss of Ecd, or because of the ovo^D1 mutation. (C,D) Mosaic egg chambers containing both Ecd^– and Ecd⁺ nurse cells were identified with the anti-Ecd antibody three days after ecd² clones had been induced in adult females. Only such mosaic egg chambers formed vitellogenic oocytes (asterisk). (C,C') Confocal sections through the same egg chamber.