Fig. 2. Consequences of epidermal Rb ablation in vivo. (A) External appearance of P8 mice with epidermal-specific Rb loss. (B-G) Haematoxylyn-Eosin (H/E) stained skin sections from Rb^F19/F19 (B), Rb^F19/F19;p107^–/– (C), Rb^F19/+;p107^–/–;K14cre (D), Rb^F19/F19;K14cre (E), Rb^F19/F19;p107^+/–;K14cre (F) and Rb^F19/F19;p107^–/–;K14cre (G) mice at P8. There are no alterations in mice bearing one functional copy of Rb and the hyperplasia and hyperkeratosis promoted by epidermal pRb loss is evident, which becomes more progressive with the concomitant loss of p107. (H) Quantitative analysis of epidermal hyperplasia measured by epidermal thickness (in µm) taken from three different aged-matched mice of each genotype counting 2-4 sections in each (mean±s.d.). Scale bars: 50 µm.