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Fig. 2. Gene expression analysis. (A) RNA from EBs differentiated either in serum (FCS) or in serum-free conditions (SR), was subjected to semi-quantitative RT-PCR. Numbers on top indicate days of EB differentiation. un, undifferentiated ES cells. (B) EBs were generated in serum-free conditions (SR) or SR+BMP4, collected at different time points, and RNA was subjected to semi-quantitative RT-PCR. Numbers on top indicate days of differentiation. N, negative H2O control.