Fig. 6. MKP3 is required in the early embryo for establishment of axial polarity. (A,D,F,H,J,L) Control MO (contMO) or 5 bp mismatch mkp3 MO (misMO)-injected embryos. (B,C,E,G,I,K,M) mkp3MO-injected embryos. Injection of mkp3MO results in dorsalized embryos (compare B and E with A and D). (C) The mkp3MO effects can be rescued by co-injection of HA::mkp3 that is insensitive to mkp3MO. (F-M) mkp3MO disrupts early dorsoventral patterning of the embryo, as shown by suppression of bmp4 (compare F with G), and expansion of chordin and fgf8 (arrows) (compare I with H and K with J). Neural markers en3 and krox20 are expanded in mkp3MO-injected embryos (compare L with M).