Fig. 1. Ectopic expression of Wnt7a promotes neuronal differentiation in cortical NPCs. Dissociated cortical NPCs were infected with a retrovirus encoding either GFP alone (control) or both GFP and Wnt7a. The cells were then incubated for 3 days in the absence of Fgf2. (A) The percentage of TuJ1⁺ cells among GFP⁺ cells was determined. Data are the mean±s.e.m. of values from three samples. Similar results were obtained in four independent experiments. ^*P<0.025 versus control; t-test. (B) GFP fluorescence, TuJ1 immunofluorescence and the corresponding merged images are shown for typical fields of control and Wnt7a-expressing cells. (C) The percentage of GFAP⁺ cells among GFP⁺ cells was determined. Data are the mean±s.e.m. of values from three samples. Scale bar: 25 µm.