Fig. 2. (A) NS1 and NS2 protein sequence compared with PRS of Arabidopsis and the closest rice relative. Note the very high similarity of the homeodomain (underlined) and the high sequence conservation between the maize and rice proteins at the C terminus. (B) Phylogeny of NS/PRS proteins. The NS proteins are more similar to PRS than they are to WUSCHEL, or to other WUSCHEL-like proteins from Arabidopsis thaliana. The unrooted tree was generated using the neighbor-joining method from a CLUSTALW alignment of the homeodomain. Bootstrap values were calculated from 1000 replicates. The maximal parsimony method yielded a phylogenetic tree with identical topology (bootstrap values calculated from 1000 replicates are shown in parentheses). AtPRS (Arabidopsis thaliana, BAB79446), AtWUS (Arabidopsis thaliana, CAA09986), AtHD (Arabidopsis thaliana, NP_188428), OsHD1 (Oryza sativa, CL042143.26.34) and OsHD2 (Oryza sativa, BAA90492).