Fig. 4. TAT-C3 treatment affects axonal morphology. The individual morphology of neuronal processes from E12 explants could be visualized after GFP electroporation. Control neurons showed straight axons (A), whereas TAT-C3-treated neurons exhibited tortuous axons (B). For quantification analysis, the trajectory of entire axons in control (C, n=11) and TAT-C3 treated explants (E, n=11) was drawn, and the mean deviation from a straight line measured (D). Data are presented using arbitrary units; ^*P<0.001. Scale bar in A: 100 µm for A,B.