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Fig. 7. Inhibition of Bmp signalling restores the ectodermal DV patterning in Fgf-depleted embryos. Analysis of the DV patterning of the ectoderm with foxi1 (A-D), cyp26a (E-H) and vhnf1 (I-L). Black arrows indicate the space between the blastoderm margin and the ectodermal gene expressions. (B) Microinjection of dn-ras causes an expansion of foxi1 expression, (F) a reduction of the neural expression domain of cyp26a (white arrowhead) and (J) a complete loss of vhnf1. (C) Co-injection of nog1 with dn-ras abolishes epidermal cell fates, (G) expands the anterior neurectoderm but (K) does not rescue vhnf1 expression. (D) nog1 injection abolishes foxi1 expression, while the neural expression domains of cyp26 (H) and vhnf1 (L) expand ventrally. (A-H) 75% epiboly stage; (I-L) 85% epiboly stage. All embryos are shown in lateral view, dorsal towards the right.