Fig. 3. Differentiation of gonad sheath cells is not affected by par-3(RNAi). (A,B) Expression of CEH-18 protein. Gonads were dissected 48 hours after growth with (right) or without (left) par-3 RNAi treatment, and stained with anti CEH-18 antibodies. Sheath nuclei are indicated by arrowheads. The size difference in the sheath nuclei in the gonads shown was not reproducible and might have been an artifact of fixation. (C,D) Distribution of myosin heavy chain A (MHCA) in gonad sheath cells of par-3(RNAi) worms. Worms were collected after 48 hours of growth with (right) or without (left) RNAi treatment and localization of MHCA was analyzed by epifluorescence microscopy. DNA was stained by DAPI. Sheath cells are numbered and sheath nuclei are indicated by numbered arrowheads; gray arrowheads indicate out of focus nuclei. Scale bar: 10 µm.