Fig. 4. Specification of spermatheca is not affected by par-3 RNAi, but distal cell morphology and tissue organization are abnormal. let-502::gfp worms were collected after 48 hours of growth with (C,D) or without (A,B) RNAi treatment, and fixed and stained with rhodamine-phalloidin. The number, shape and distribution of cells with high levels of GFP in the distal end of spermatheca was compared. (A,C) Projections of 20 adjacent optical sections. Note the more spherical shape of cells in (C) and the gap between GFP-positive cells in (D') (arrow). Such gaps are not seen in controls. (E) Summary of the results of the tissue organization analysis. Scale bar: 5 µm.