Fig. 6. Time course of PAR-3 and AJM-1::GFP expression. Worms carrying AJM-1::GFP were collected 27 hours (A), 30 hours (B), 33 hours (C), or 36 hours (D) after incubation. Localization of PAR-3 was examined by immunohistochemistry. Multiple sections were stacked in each panel for analysis. PAR-3 was detected in vulval precursor cells (vulva), uterine precursor cells (ut), spermathecal precursor cells (sp) and sheath cells (arrow in B'). PAR-3 levels begin to decrease in 36-hour-incubated worms (arrow in D'). Scale bar: 5 µm.