Fig. 7. Effect of par-3 RNAi on expression of PAR-3, PAR-6 and PKC-3. Worms were collected after 30 hours of growth with (right) or without (left) RNAi treatment. Localization of PAR-3 (A,B), PAR-6 (C,D) and PKC-3 (E,F) was examined by immunohistochemistry. Multiple optical sections were stacked in each panel for analysis. The vulval precursor cells (vulva), uterine precursor cells (ut), and spermathecal precursor cells (sp) are indicated. The amounts of all three proteins were significantly reduced in the distal part of the spermathecal primordium (arrows) where cells had not been polarized (arrowheads), as indicated by the absence of apical enrichment of HMP-1::GFP. (L). Expression of PAR-3 in par-3(it71) homozygous worms after 30 hours of growth on OP50. Scale bar: 5 µm.