Fig. 5. Blocking vß3 integrin activity disrupts early vasculogenesis. (A-C) Taken from Movie 5 (see http://dev.biologists.org/supplemental), show changes in endothelial structures over a 10 hour period within an embryo injected with LM609, an vß3 blocking antibody. (D) The merged image of the Cy3-QH1, pulse labeled (red) and the post-experimentally labeled vasculature (green). In D, the disruption of the dorsal aortae and the polygonal network is clearly visible (see Fig. 1 as a normal reference). Clusters of vascular endothelial cells, characteristic for earlier stages (A in Figs 1 and 5) persist, lacking protrusions and connections. The red and green open circles indicate the same endothelial structures on each panel. By comparing with Fig. 1, a substantial reduction in the lateral-to-medial vascular drift is observed (red bars). The vasculature marked by green open circles is fused while being drawn into the developing endocardium. This motion seems to be unaffected by LM609 exposure. The cyan box delineates the field that is enlarged in Fig. 6 and serves as a frame of reference between panels. The position of this area as well as the markers, with the exception of one (is, injection site), is the same as in Fig. 1.