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Fig. 4. Both reduction of nmo and ectopic nmo can affect Wg-dependent gene expression. (A) Dll-lacZ expression is seen in a broad domain centered on the DV boundary with areas of increased expression at the anterior and posterior edges of the DV boundary. (B) Ectopic Nemo in ap>nmo can greatly reduce the Dll-lacZ expression, particularly in the posterior margin region. Dll expression is enhanced in nmo mutant clones. (C,D) nmoDB24 clones (marked by the absence of GFP, green). (D,E) Expression of Dll-lacZ (anti ß-gal, red) is also increased in nmoDB24 clones (arrow in E).