Fig. 6. Wg signaling positively regulates the expression of nmo. (A) vg-Gal4 is expressed along the DV boundary in vg>lacZ. (B) nmo expression in vg-Gal4/UAS-fluarm; nmo-lacZ/+ mid third instar larval discs is greatly expanded, especially in the posterior periphery. (C) dpp>fluarm causes ectopic nmo expression along the AP boundary. (D-F) nmo-lacZ expression (E,F; anti ß-gal, red) in fluarm flip-out clones (D,E; marked by the areas of brighter GFP staining). (G-I) Flip-out clones ectopically expressing UAS-Daxin (G,H) also result in decreased nmo expression (H,I). (J-L) In dsh^v26 somatic clones (J,K; marked by the absence of GFP, green) nmo-lacZ expression is reduced cell autonomously (K,L; anti-ß-gal, red).