Fig. 7. Nemo can influence Arm stabilization. (A,B) nmo gene expression (as monitored by nmo-lacZ, anti ß-gal, red) overlaps with stabilized Arm protein (B,C; anti-Arm, green) in third instar discs. There are distinct regions in which higher nmo expression in A excludes high levels of Arm (arrow in C) and in which high levels of Arm seen in C coincide with reduced nmo (arrowhead in A). In flip-out clones ectopically expressing Nemo (D,E; marked by the areas of brighter GFP staining), the stabilization of Arm protein appears reduced in a cell-autonomous manner (E,F; anti-Arm, red; arrows in F).