Fig. 7. gld-3 mog-6 double mutants have tumorous germlines. Adult hermaphrodites were analyzed for germline phenotypes by DIC. (A) gld-3(RNAi) caused feminization of mog-6/+ heterozygotes (arrow points toward a stacking oocyte). (B) Germlines of mog-6 homozygotes were masculinized (sperm is shown between the two arrows). (C) gld-3(RNAi) mog-6 hermaphrodites are tumorous. (D) Dissected tumorous germline stained for DNA (top) or for the mitotic marker phosphohistone H3 (PH3) (bottom). Mitotic germ cells are present throughout the entire germline, including the proximal part of the germline (white arrowheads). The distal end is on the left. (E) Magnification of the proximal region shown in D (top) in a different focal plane. Asterisks indicate distal ends of germlines. Black arrowheads point toward the vulva. Scale bar: 20 µm.