Fig. 5. Analysis of LvDsh domains required for vegetal, cortical localization (VCL). Mutant constructs and their designations are indicated. `+' indicates that VCL was evident in essentially all embryos oriented favorably; `+/–' indicates that some favorably oriented embryos, but not all, exhibited VCL. In addition, in those embryos in which VCL was apparent, the crescent of Dsh-GFP was generally less pronounced than observed following injection of mRNA encoding wild-type LvDsh-GFP. `–' indicates that essentially no embryos with unambiguous VCL could be identified.