Fig. 4. Gametophytic maternal effect mutants with a fis phenotype. KS117 GFP is misexpressed. In mutant seeds, the level of GFP accumulation is much higher and the expression is not restricted to the posterior pole in mutant seeds. More than 25% of seeds abort. One line from each mutant group is presented here. (A-G) Seeds observed by epifluorescence stereomicroscopy. (H-N) Seeds observed by light microscopy. All aborting seeds are indicated by red dots. (A,H) WT KS117; (B,I) dme-4/DME; (C,J) mea-6/MEA; (D,K) fis2-6/FIS2; (E,L) fie-11/FIE; (F,M) bga-1/BGA; (G,N) msi1-2/MSI1. Scale bar: 200 µm (all images).