Fig. 3. Gene expression patterns in kan1 kan2 kan3 and AS1>>KAN2 leaves. (A) GUS activity, due to an enhancer trap in the YAB3 gene, is localized to the abaxial regions of developing leaves (arrowheads) in yab3-2 plants. (B) In contrast, no detectable GUS activity is detected in kan1-2 kan2-1 kan3-1 yab3-2 plants. (F) In wild-type plants PHB expression is localized to the apical meristem and the adaxial regions of lateral organs. (C,D) In a kan1 kan2 kan3 background, PHB expression is no longer adaxially confined in developing leaves, but still displays a gradient with the highest level adaxially. (E) When KANADI activity is expressed uniformly throughout leaves, such as when KAN2 is driven by the AS1 promoter, the leaves are both radialized with no lamina formation and abaxialized. (G,F) PHB expression is not detected in AS1>>KAN2 leaves (G) whereas PHB is expressed adaxially in wild-type leaves (F). (H) FIL is expressed abaxially in wild-type leaf primordia, and its expression becomes localized to the abaxial marginal regions as leaves differentiate. (I) In contrast, FIL is only transiently expressed in the radial abaxialized AS1>>KAN2 leaf primordia. ab, abaxial; ad, adaxial; lp, leaf primordium; m, meristem.