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Fig. 1. Large glycogen inclusions are observed in 1-month-old Gaa-/- tissues. (A-D) Histological sections of 1-month-old Gaa-/- heart (A), skeletal muscle (B), diaphragm (C) and liver (D) were stained for glycogen with PAS. Similar tissues from age-matched normal C57B6/129-SvJ animals were included as controls (E-H). (I-P) Electron micrographs of single cells from Gaa-/- (I-L) and normal (M-P) heart (I,M), skeletal muscle (J,N), diaphragm (K,O) and liver (L,P). Scale bars: 20 µm (A-H); 500 µm (I-P).