Fig. 6. Vector is detected in in utero rAAV1-CMV-hGAA-transduced diaphragms by QC-PCR. Quantitative-competitive PCR was performed to determine vector genome copies in in utero vector-treated and untreated diaphragm samples that were previously analyzed in Fig. 4. PCR was performed after mixing increasing amounts of competitor DNA (10⁰ to 10⁸ copies) and 200 ng of total DNA isolated from the diaphragms of Gaa^-/- untreated mice or Gaa^-/- mice in utero-treated with 8.14x10¹⁰ particles of rAAV1-CMV-hGAA. Representative samples (3 of the 8 assayed) are listed, with their respective values representing estimated vector copies per diploid genome and average GAA activity. Raw QC-PCR data from each diaphragm is shown on the far right.