Fig. 7. In utero delivery of rAAV2-CBA-hGAA preserved diaphragm muscle contractile force in Gaa^-/- mice. A dose of 2x10⁸ infectious particles of rAAV2-CBA-hGAA was delivered in utero to Gaa^-/- mice (black triangles; n=3). Animals were sacrificed and diaphragm muscle strips were prepared 6 months postpartum. In vitro isometric force-frequency relationships were obtained and compared with those of age-matched, untreated normal C57B6/129-SvJ (black squares; n=3) and Gaa^-/-(white circles; n=6) mice.