Fig. 2. XStau is expressed in Xenopus oocytes. (A) Total oocyte S10 lysate was immunoblotted with either preimmune sera (lane 1) or XStau antibodies (lane 2). A single band of 80 kDa is recognized by XStau antibodies (lane 2). Molecular weight markers are indicated at the left. (B) Oocyte S10 lysates were prepared from each stage of oogenesis [I-VI (Dumont, 1972)]. One oocyte equivalent of S10 lysate per lane was resolved by SDS-PAGE and immunoblotted using XStau antibodies (above) or tubulin antibodies (below).