Fig. 5. XStau RNP complex contains Vg1 and VegT RNA. (A) Oocyte S10 lysate was fractionated on a 5-40% sucrose velocity gradient. Fractions were collected, resolved by SDS-PAGE and immunoblotted for anti-XStau (top) and anti-rpS6 (bottom). The bracket denotes the 20S XStau RNP population, and the positions of size markers are indicated at the bottom. (B) Gradient fractions containing the 20S XStau complex (A, bracket) were pooled, formaldehyde crosslinked and immunoprecipitated with XStau antibodies. The resulting RNA was amplified by RT-PCR (lanes 2, XStau) with specific primer sets for Vg1, VegT, Xcat2, Xwnt11 and EF1. A control non-relevant antibody was used to determine background interactions (lanes 1, IgG), and input control for each primer set was determined from total oocyte RNA (lanes 3, T).