Fig. 6. XStau associates with a kinesin motor. (A) Oocyte S10 lysate was fractionated on a 5-40% sucrose gradient. Fractions were collected, resolved by SDS-PAGE and immunoblotted with XStau (top), kinesin (middle), and dynein (bottom) antibodies. The positions of size markers are indicated at the bottom. (B) Immunoprecipitation was carried out using oocyte S10 lysate with beads only (lanes 1, 3), anti-XStau (lane 2) or SUK4 (kinesin) antibodies (lane 4). After SDS-PAGE, the immunoprecipitates were immunoblotted with either SUK4 (top) or XStau (bottom) antibodies.