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Fig. 2. Imaging of mitochondrial autofluorescence. (A-C) Simultaneous imaging of FAD++ autofluorescence (green, A) and mitochondria using the potentiometric dye TMRM (red, B). Merging the image of mitochondria and of autofluorescence (C) clearly shows that the FAD++ autofluorescence colocalises with mitochondria. (D-F) Simultaneous imaging of FAD++ (D, green) and NADH (E, blue). Merging the NADH and FAD++ images (F) shows that a significant part of the NADH signal is cytosolic. MS, meiotic spindle.