Fig. 3. Oscillations in FAD⁺⁺ autofluorescence are stimulated by fertilisation induced Ca²⁺ transients. (A) Variations in [Ca²⁺]_c (measured with indo 1 AM, blue trace) and FAD⁺⁺ autofluorescence (green trace) observed at fertilisation of a mouse egg (n=20). Time 0 corresponds to the time of insemination. (B) Variations in [Ca²⁺]_i (measured with rhod 2 AM, red trace) and FAD⁺⁺ autofluorescence (green trace) in a mature mouse egg injected with caged Ins(1,4,5)P₃ (n=10). A UV flash (red arrowhead) releases Ins(1,4,5)P₃ in the egg and triggers a Ca²⁺ transient accompanied by a transient decrease in FAD⁺⁺ autofluorescence. i and ii show the same egg as in Fig. 2D-F with the FAD⁺⁺ signal (i) and Rhod2 AM (ii) signal that have markedly different distributions. This suggests that, under our conditions, rhod2 AM does not partition into mitochondria of the mouse egg. The ROI (white circle in i) used to obtain the measurements is drawn. (C) Oscillations of FAD⁺⁺ autofluorescence are stopped by the addition of 5 µM BAPTA AM to the chamber (the presence of BAPTA AM is indicated by a bar under the graph, n=8)