Fig. 7. Mitochondrial inhibitors cause an increase in resting [Ca²⁺]_i in mature eggs. (A,B) Increase in [Ca²⁺]_i induced by addition to an unfertilised egg of FCCP (1 µM) in normal H-KSOM medium (A) or in H-KSOM without Ca²⁺ and supplemented with 2 mM EGTA (B). (C,D) Increase in [Ca²⁺]_i induced by addition to an unfertilised egg of CN^– (2 mM) in normal H-KSOM medium (C) or in H-KSOM without Ca²⁺ and supplemented with 2 mM EGTA (D). (E,F) Variations of [Ca²⁺]_i induced by sequential addition to an unfertilised egg of 20 µM thapsigargin and FCCP (1 µM) in normal H-KSOM (E) or in H-KSOM without Ca²⁺ (F).