Fig. 8. Mitochondrial inhibitors cause an increase in resting [Ca²⁺]_c by depleting ATP levels in mature eggs. (A) Simultaneous measurement of [Ca²⁺]_i (using fura 2 AM) and of m [using Rhod 123 (compare with Materials and methods)] in mature eggs. Where indicated, oligomycin (60 µM) and FCCP (1 µM) are added to the bath. (B) Photon emission of a single egg injected with luciferase. Where indicated, oligomycin (60 µM) and FCCP (1 µM) are added to the bath. The background luminescence count was of six photons per 10 seconds in this experiment. (C) Schematic representation of a mitochondria and the effect of oligomycin on the proton fluxes generated at the F₀/F₁ synthase.